Tackling Formalin-Fixed, Paraffin-Embedded Tumor Tissue with Next-Generation Sequencing
نویسندگان
چکیده
منابع مشابه
Tackling formalin-fixed, paraffin-embedded tumor tissue with next-generation sequencing.
Most tumor samples available for clinical genotyping are formalin-fixed and paraffin-embedded (FFPE), but there has been relatively little published on the suitability of such samples for next-generation sequencing approaches. A new study by Wagle and colleagues shows that a combination of hybridization-capture and deep sequencing yields high-quality data from FFPE specimens.
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Next Generation Sequencing (NGS) technologies are used to detect somatic mutations in tumors and study germ line variation. Most NGS studies use DNA isolated from whole blood or fresh frozen tissue. However, formalin-fixed paraffin-embedded (FFPE) tissues are one of the most widely available clinical specimens. Their potential utility as a source of DNA for NGS would greatly enhance population-...
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ژورنال
عنوان ژورنال: Cancer Discovery
سال: 2012
ISSN: 2159-8274,2159-8290
DOI: 10.1158/2159-8290.cd-11-0319